| 产木聚糖酶白地霉培养特性及部分纯化的酶学特性 |
| Growth conditions and xylanase property of Geotrichum candidum |
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| 中文关键词:低温菌，木聚糖酶产酶条件，最佳生长条件 |
| 英文关键词:psychrophile, xylanase producing condition, best growing condition |
| 基金项目:青海省社会发展及基础研究资助项目（2008 No. Z-608） |
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| 中文摘要: |
| 本文对白地霉Ref1的培养特性、产酶条件和酶学特性进行了初步研究。结果表明：该菌为低温型菌株，其最佳生长条件为pH6、20℃和酵母膏作为氮源；最佳产酶条件为pH3－7、15℃及以酵母膏氮源；条件优化后产酶可达118.7U/mL，可溶蛋白含量可达到60?g/mL，酶溶液的比活可达到1250U/mg蛋白质；该木聚糖酶的最适反应温度和pH分别为50℃和5，金属离子Mg2+、Na+和8mmol/L的Fe2+、Cu2+、Zn2+等对木聚糖酶的活性有抑制作用，而 Ca2+、4mmol/L的Fe2+、Cu2+、Zn2+和8mmol/L的Mn2+等对该酶反应则有促进作用；该木聚糖酶在保温2h后在15－40℃范围内能保持80%以上的酶活性，在50℃时能保持68%的酶活性；用 lineweaver-Burk作图法（双倒数作图法）求得该酶的最大反应速度Vmax和Km值分别为163.38mmol/mg/min和0.75mg/mL。 |
| 英文摘要: |
| The characteristics of culture, xylanase producing condition and characteristics of enzyme of Geotrichum candidum Ref1 were studied. The result showed that Ref1 was a psychrophile, and the best growing conditions were pH 6, 20℃ and yeast extract as nitrogen source while the best xylanase producing conditions were pH 3－7, 15℃ and yeast extract as nitrogen source. Xylanase activity of optimized Ref1 reached 118.7U/mL and soluble protein 60?g/mL; the specific activity of the crude enzyme was 1250U/mg protein. The xylanase has an optimal activity at 50℃ and pH 5.0, and the relative enzyme activity remains 80% while keeping at the temperatures of 15－40℃. Mg2+, Na+ and 8mmol/L Fe2+, Cu2+, Zn2+ were strong inhibitors, while Ca2+ , 4mmol/L Fe2+, Cu2+, Zn2+ and 8mmol/L Mn2+ were stimulators of the enzyme. The maximum velocity of reaction and the Km of the xylanase were 163.38mmol/mg/min and 0.75mg/mL, respectively. |
| 谢占玲 汪永录 赵国珍 张 曼 侯 辉 朱海梅 刘 英 吴 润. 产木聚糖酶白地霉培养特性及部分纯化的酶学特性[J]. 菌物学报, 2009, 28(2): 253-260 |
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