| 木腐菌氧化酶系检定及漆酶产生的研究 |
| Ligninolytic enzyme system examination and laccase production of wood decay fungi |
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| 中文关键词:木素氧化酶系，木蹄层孔菌，培养方式，染料脱色 |
| 英文关键词:lignin oxidative system, Fomes fomentarius, cultivation method, dye decolorization |
| 基金项目:世界自然基金（WWF，CN0078.01）；国家自然科学基金资助项目（No. 30170775，No. 30671702） |
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| 中文摘要: |
| 采用丁香醛连氮、苯胺蓝平板脱色等4种方法，定性检测了27种真菌的木素氧化酶系组成。结果表明5个菌种同时具有漆酶（Lac）、木素过氧化物酶（Lip）和锰过氧化物酶（Mnp）3种酶的活性，5个菌种同时具有Lac和Lip 2种酶的活性，8个菌种具有1种酶的活性；12个菌种具有Lac活性。选择其中生长速度快、Lac活性高的5个菌种进行Lac产生的研究，发现木蹄层孔菌Fomes fomentarius诱导、静止培养条件下产生的Lac酶活峰值高达9496U/mL，远远高于其它已报道过的菌种；静止培养条件下贝形刺革菌Hymenochaete badio-ferruginea和乳白耙菌Irpex lacteus产Lac，其峰值也分别达到了652和292U/mL，均明显高于其它培养方式，说明木蹄层孔菌等3种真菌静止培养可代替振荡培养进行Lac产生、制备等相关的后续研究。木蹄层孔菌等5种真菌对刚果红等染料脱色作用研究表明，各菌种对4类染料均具有高效广谱的脱色作用。 |
| 英文摘要: |
| The lignin oxidative system of 27 fungal species were analyzed qualitatively by four methods including using syringaldazine as substrate and aniline blue decolorization in plate cultivation. The result showed that 5 species had laccase (Lac), ligin peroxidase (Lip) and manganese peroxidase (Mnp), and 5 species simultaneously had Lac and Lip. Other 8 species only had one enzyme activity. Twelve species produced Lac. Lac activity of Fomes fomentarius reached a peak of 9496U/mL in inducted and static cultivation, which was obviously higher than that of other fungi. By static cultivation, the Lac activity of Hymenochaete badio-ferruginea and Irpex lacteus reached a peak of 652 and 292U/mL, respectively, which were higher as compared with other cultivation methods. It proved that static cultivation could take the place of shaking cultivation in the production and preparation of Lac of Fomes fomentarius, Hymenochaete badio-ferruginea and Irpex lacteus. In the decolorization assay, it was found that Fomes fomentarius, Hymenochaete badio-ferruginea, Irpex lacteus, Phellinus igniarius and Trichaptum fusco-violaceum had efficient and broad-spectrum discolored function to congo red, alizarin red, methylene blue and crystal violet. |
| 赵 敏 朱 琳 魏 巍. 木腐菌氧化酶系检定及漆酶产生的研究[J]. 菌物学报, 2009, 28(2): 244-252 |
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