| 深层培养的斜生褐孔菌酚类化合物对铅处理小鼠耐力的增强作用 |
| Phenolic compounds from submerged cultures of Phaeoporus obliquus enhance tolerance of lead-treated mice |
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| 中文关键词:硬毛素类，黄酮类，黑色素，常压缺氧和游泳的耐力，抗氧化 |
| 英文关键词:hispidin analogs, flavonoids, melanins, tolerance for atmospheric hypoxia and swimming, antioxidant activity |
| 基金项目:Natural Science Foundations of Education Department of Jiangsu Province (No. 05KJA36012) and a grant of Natural Science Foundations from the Government of Jiangsu Province (No. BK2006034) |
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| 中文摘要: |
| 本文研究了不同条件下深层培养的斜生褐孔菌Phaeoporus obliquus与野生桦褐孔菌在酚类化合物组成及其含量上的差异以及对铅处理小鼠常压缺氧和游泳耐力影响。野生桦褐孔菌主要含有水溶性黑色素、硬毛素衍生物，两者各占总酚类化合物的12.1%和42.2%。而深层培养的桦褐孔菌则主要积累黄酮类化合物，其中正常生理条件下积累量达到总酚的35.5%，加入H2O2后积累量达到总酚的92.5%，同时加入H2O2和熊果苷后则为总酚的76.9%。正常培养基中水溶性黑色素的积累量达到总酚的20.2%，而加入H2O2或同时加入H2O2和熊果苷后水溶性黑色素则很少合成。口服野生斜生褐孔菌或不同条件下深层培养的斜生褐孔菌酚类化合物都能使铅处理小鼠忍耐缺氧和游泳的时间延长12–15min。同时，口服不同来源的斜生褐孔菌酚类化合物可提升铅处理小鼠外周淋巴细胞的活力和总超氧化物歧化酶活性，并降低丙二醛的积累。因此，斜生褐孔菌酚类化合物对铅处理小鼠耐缺氧和游泳的提升作用在于抑制了脂质过氧化反应，增强了过氧化物歧化酶的表达，从而保护了铅处理小鼠的细胞和器官免于氧化胁迫的损伤。 |
| 英文摘要: |
| This study is aiming at clarifying the differences in the content of phenolic compounds between Phaeoporus obliquus grown in natural habitats and that in continuously stirring tank reactor (CSTR) under different conditions and their effects on tolerance for atmospheric hypoxia and swimming in lead-treated mice. P. obliquus grown in natural habitats produces mainly soluble melanins and hispidin analogs (HA), containing 12.1% and 42.2% of the total phenolic compounds (TP), respectively, while that grown in CSTR accumulates predominantly flavonoid aglycones, accounting for 35.5% of TP in control medium, 92.5% in H2O2-added medium and 76.9% in H2O2-abutin-added medium. Melanins in control medium reached 20.2% of TP, but those from the media with H2O2 addition or simultaneous addition of H2O2 and arbutin were less synthesized. Oral administration of phenolic compounds from the fungus grown in its natural habitats and CSTR all extended 12–15min for tolerating atmospheric hypoxia and swimming in lead-treated mice. Oral treatment of these compounds also enhanced viability of peripheral lymphocytes and the activity of T-SOD, but reduced malondialdehyde (MDA) in lead-treated mice. Thus enhanced tolerance for hypoxia and swimming might be attributed to the inhibition of lipid peroxidation and enhanced expression of SOD by TP from the fungus grown both in natural habitats and CSTR, and thereby protecting cells and organs in mice from lead-induced oxidative damage. |
| 郑维发 殷兆娟 陈才法 张梅梅 赵艳霞. 深层培养的斜生褐孔菌酚类化合物对铅处理小鼠耐力的增强作用[J]. 菌物学报, 2009, 28(1): 112-119 |
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