| 中国被毛孢发酵液中一种镇静催眠活性物质的分离纯化和结构鉴定 |
| Isolation and identification of sedative and hypnotic compound from the fermentation broth of Hirsutella sinensis |
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| 中文关键词:活性指导下的分离，茶碱-9-葡萄糖苷 |
| 英文关键词:bioactive guided isolation, 9-glucopyranosyl theophylline |
| 基金项目:国家自然科学基金项目（No. 30570189） |
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| 中文摘要: |
| 本研究对中国被毛孢Hirsutella sinensis菌株RCEF0273发酵液的乙酸乙酯粗提物中的镇静催眠活性物质进行了分析。通过活性指导下的色谱分离，从乙酸乙酯提取物中得到了一种白色晶体化合物，质谱（MS）和核磁共振（NMR）的鉴定结果表明该化合物为茶碱-9-葡萄糖苷。活性试验显示该化合物可抑制小鼠的自发活动，缩短小鼠入睡潜伏期，延长小鼠睡眠持续时间，具有镇静催眠活性。 |
| 英文摘要: |
| The acetylacetate extract of the fermentation broth of Hirsutella sinensis RCEF0273 having sedative and hypnotic activity was analysed. By means of bioactive guided isolation of column chromatography and high performance chromatography, a white pure compound was obtained, and the compound was identified as 9-glucopyranosyl theophylline by the methods of mass spectroscopy (MS) and nuclear magnetic resonance (NMR). Sedative and hypnotic activity of 9-glucopyranosyl theophylline was traced, and the results showed the compound significantly inhibited the spontaneous activity of rats. Moreover, the compound shortened the sleep latency, and prolonged the duration of sleep of rats. |
| 丁 婷,邵 颖,樊美珍. 中国被毛孢发酵液中一种镇静催眠活性物质的分离纯化和结构鉴定[J]. 菌物学报, 2008, 27(6): 956-963 |
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