细脚拟青霉菌丝体多糖纯化及组成分析 |
Purification and component analysis of polysaccharides from mycelia of Paecilomyces tenuipes |
|
中文关键词:纤维素柱,葡聚糖凝胶柱,红外光谱 |
英文关键词:DEAE-cellulose chromatogram, sephadex chromatogram, infrared spectrum |
基金项目: |
|
摘要点击次数: 3109 |
全文下载次数: 3288 |
中文摘要: |
通过DE-52纤维素柱和Sephedex G-100葡聚糖凝胶柱,对细脚拟青霉Paecilomyces tenuipes菌丝体粗多糖进行纯化,得到PtPs1和PtPs2,应用离子色谱HPAEC-PAD对纯多糖PtPs1和PtPs2的单糖组成进行分析,证实均由葡萄糖、半乳糖和甘露糖三种单糖组成,经红外扫描确定PtPs1和PtPs2均为α型吡喃糖。 |
英文摘要: |
The polysaccharides of Paecilomyces tenuipes mycelia were purified by using DEAE cellulose DE-52 and Sephedex G-100, and pure polysaccharides PtPs1 and PtPs2 were obtained. The structural unit (monosaccharide) of PtPs1 and PtPs2 was analyzed by HPAEC-PAD. The results indicated that both PtPs1 and PtPs2 consisted of glucose, galactose and mannose. The infrared spectral analysis showed that PtPs1 and PtPs2 were all pyranose of α-configuration. |
刘桂君,文华安. 细脚拟青霉菌丝体多糖纯化及组成分析[J]. 菌物学报, 2008, 27(6): 947-955 |
查看全文 查看/发表评论 下载PDF阅读器 |
|