细脚拟青霉菌丝体多糖纯化及组成分析
Purification and component analysis of polysaccharides from mycelia of Paecilomyces tenuipes
  
中文关键词:纤维素柱,葡聚糖凝胶柱,红外光谱
英文关键词:DEAE-cellulose chromatogram, sephadex chromatogram, infrared spectrum
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作者单位
刘桂君 中国科学院微生物研究所真菌地衣系统学重点实验室 北京 100101 北京顺鑫农业股份有限公司牛栏山酒厂 北京 101301 
文华安 中国科学院微生物研究所真菌地衣系统学重点实验室 北京 100101 
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中文摘要:
      通过DE-52纤维素柱和Sephedex G-100葡聚糖凝胶柱,对细脚拟青霉Paecilomyces tenuipes菌丝体粗多糖进行纯化,得到PtPs1和PtPs2,应用离子色谱HPAEC-PAD对纯多糖PtPs1和PtPs2的单糖组成进行分析,证实均由葡萄糖、半乳糖和甘露糖三种单糖组成,经红外扫描确定PtPs1和PtPs2均为α型吡喃糖。
英文摘要:
      The polysaccharides of Paecilomyces tenuipes mycelia were purified by using DEAE cellulose DE-52 and Sephedex G-100, and pure polysaccharides PtPs1 and PtPs2 were obtained. The structural unit (monosaccharide) of PtPs1 and PtPs2 was analyzed by HPAEC-PAD. The results indicated that both PtPs1 and PtPs2 consisted of glucose, galactose and mannose. The infrared spectral analysis showed that PtPs1 and PtPs2 were all pyranose of α-configuration.
刘桂君,文华安. 细脚拟青霉菌丝体多糖纯化及组成分析[J]. 菌物学报, 2008, 27(6): 947-955
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