| 葡萄生轴霜霉菌孢子囊的长期保存 |
| Long-term preservation methods for sporangia of Plasmopara viticola |
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| 中文关键词:二甲基亚砜，低温，致病性，发病率 |
| 英文关键词:dimethylsulfoxide, low temperature, pathogenicity, disease rate |
| 基金项目:河北农业大学科研启动基金资助 |
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| 中文摘要: |
| 为了解决葡萄生轴霜霉菌的活体保存问题，本文开展了多种保藏方法的研究。采用二甲基亚砜和甘油分别于不同低温条件下对孢子囊进行了保存，取出后进行接种试验。结果表明，孢子囊用不同保护剂处理后保存于-76℃中5d，10%二甲基亚砜处理的孢子囊经接种后发病面积百分率为68.3%，其致病性明显强于25%甘油处理；孢子囊用不同浓度的二甲基亚砜保存于-76℃中30d，5%和10%二甲基亚砜处理后致病性差异不显著，但明显高于15%、20%、25%二甲基亚砜处理的孢子囊；5%和10%二甲基亚砜处理的孢子囊，在-76℃中保存5d和15d后，致病性明显高于-20℃的处理；孢子囊经-76℃保存240d后，10%二甲基亚砜处理的孢子囊无致病性，而5%二甲基亚砜处理的发病面积百分率为56.3%，与对照无显著差异，说明孢子囊用含5%的二甲基亚砜处理能在-76℃中长期保存。 |
| 英文摘要: |
| Different concentrations of dimethylsulfoxide and glycerol were used to preserve sporangia of Plasmopara viticola in different low temperatures. The results showed that the sporangia preserved in 10% dimethylsulfoxide were more virulent than those in 25% glycerol at -76℃. The proper concentrations of dimethylsulfoxide for preserving sporangia were 5% and 10%, and the sporangia had stronger pathogenicity than those preserved in 15%, 20% and 25% dimethylsulfoxide. The sporangia preserved in 5% and 10% dimethylsulfoxide at -76℃ for 5 days and 15 days were more pathogenic to grape leaves than those kept at -20℃. The sporangia preserved at -76℃ for 240 days in 10% dimethylsulfoxide lost their pathogenicity, while those kept in 5% dimethylsulfoxide remained virulent to grape leaves with 56.3% of infected leaf area after inoculation. |
| 杜兴兰,李正楠,姬惜珠,冉隆贤. 葡萄生轴霜霉菌孢子囊的长期保存[J]. 菌物学报, 2008, 27(6): 908-914 |
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