| 黄檗根围丛枝菌根（AM）真菌的分离与分子鉴定 |
| Molecular detection of the arbuscular mycorrhizal fungi in the rhizosphere of Phellodendron amurense |
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| 中文关键词:25S rDNA，nested-PCR，系统发育分析 |
| 英文关键词:25S rDNA, nested-PCR, phylogenetic tree |
| 基金项目:黑龙江省博士后基金（No. LBH-Z05013）；黑龙江大学博士启动基金 |
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| 中文摘要: |
| 从东北林业大学林场采集黄檗Phellodendron amurense根系及根围土壤，采用形态学和分子生物学方法对分离得到的丛枝菌根（arbuscular mycorrhiza，AM）真菌孢子进行了分类鉴定。依据AM真菌孢子形态特征鉴定出黄檗根围土壤中存在4种AM真菌：根内球囊霉Glomus intraradices，摩西球囊霉Glomus mosseae，美丽盾孢囊霉Scutellospora calospora和变形球囊霉Glomus versiforme，而应用nested-PCR技术从黄檗根内只检测到了G. intraradices，G. mosseae和Scu. calospora，表明G. versiforme未侵染黄檗根系。 |
| 英文摘要: |
| The rhizospheric soil and root samples of Phellodendron amurense were collected in the Logging Station of Northeast Forestry University. The AM fungi isolated were identified morphologically and molecularly. Four species were obtained. They were Glomus intraradices, Glomus mosseae, Scutellospora calospora and Glomus versiforme. However, only G. intraradices, G. mosseae and S. calospora were detected from the root samples of Phellodendron amurense by nested-PCR. It was indicated that the roots of Phellodendron amurense were not infected by G. versiforme. |
| 蔡柏岩,接伟光,葛菁萍,阎秀峰. 黄檗根围丛枝菌根（AM）真菌的分离与分子鉴定[J]. 菌物学报, 2008, 27(6): 884-893 |
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