| 一株产紫杉醇菌的分离及其代谢产物对HeLa细胞抑制作用的研究 |
| Isolation of a taxol-producing endophytic fungus and inhibiting effect of the fungus metabolites on HeLa cell |
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| 中文关键词:内生真菌，葡萄孢，红豆杉，发酵提取物 |
| 英文关键词:endophytic fungus, Botrytis sp., Taxus cuspidata, fermented extract |
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| 中文摘要: |
| 从东北红豆杉Taxus cuspidata的主干和侧枝树皮及皮下分离筛选得到181株纯化的内生菌株。经过摇瓶发酵培养，再用甲醇、乙酸乙酯萃取抽提，抽提产物经以标准紫杉醇为对照的薄层层析（TLC）、高效液相色谱（HPLC）、质谱（MS）分析，发现有1株菌株HD181-23能够产生紫杉醇，其紫杉醇产量为206.34μg/L。对菌株HD181-23菌落、菌丝体和孢子形态和产孢特征进行了研究，表明菌株HD181-23为葡萄孢属的种，暂定为Botrytis sp.。该菌株的发酵提取纯化产物经HeLa细胞毒实验，结果显示对HeLa细胞具有较强的抑制作用。 |
| 英文摘要: |
| One hundred and eighty one purified endophytic fungal strains were isolated and screened from inner bark (phloem-cambium) and xylem of Taxus cuspidata. These strains were fermented and then the fermented solution was extracted using methanol and ethyl acetate. The extracts were analyzed by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard taxol as control. The results showed that strain HD181-23 can produce taxol with output of 206.34μg/L. Morphological observation indicates that HD181-23 belongs to the genus Botrytis, and the fungus is tentatively identified as Botrytis sp. The purified products of the fermented extracts have significant inhibitive effects on HeLa cell. |
| 赵凯 赵立斐 金媛媛 魏海霞 平文祥 周东坡*. 一株产紫杉醇菌的分离及其代谢产物对HeLa细胞抑制作用的研究[J]. 菌物学报, 2008, 27(5): 735-744 |
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