| 构巢曲霉胞质分裂SIN信号途径中sepH的反向调节子研究 |
| Suppressors of sepH regulating cytokinesis of SIN pathway in Aspergillus nidulans |
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| 中文关键词:突变，隔膜，诱变，筛选，杂交 |
| 英文关键词:mutation, septation, mutagenesis, screening, cross |
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| 中文摘要: |
| 在构巢曲霉Aspergillus nidulans的细胞隔膜开始网（septation initiation network，SIN）的信号途径中，sepH基因对胞质分裂起着关键的正调节作用，但对该途径中同样起着十分重要作用的有关反向调节子（suppressors）的研究目前还不清楚。本课题采用UV诱导点突变法成功筛选到sepH突变株的反向调节子116株，这些突变株共分为三类。通过对突变株进行杂交和回交等遗传学分析，排除了sepH回复突变菌株，获得了能使sepH胞质分裂恢复正常的温度敏感菌株Sin110，证实了SIN途径具有对应反向的旁路途径（bypass）的存在。 |
| 英文摘要: |
| As a member of septation initiation network (SIN), sepH is a key factor of cytokinesis in Aspergillus nidulans. However, how the suppressors of sepH affect cytokinesis is not yet understood. In this study, 116 mutants which suppressed the phenotype of sepH were isolated by UV mutation. These suppressors can restore septation and conidiation in the absence of the SEPH protein. By the cross and backcross, the temperature sensitive strain Sin110 was obtained and the bypass of SIN pathway was identified. |
| 管祁 陈为为 马兆飞 徐旭士 陆玲*. 构巢曲霉胞质分裂SIN信号途径中sepH的反向调节子研究[J]. 菌物学报, 2008, 27(5): 727-734 |
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