| 硒镧复合作用对巴西蘑菇子实体形态和数量特征的影响 |
| Multiple effects of Se and La on morphological and quantitative characters of Agaricus brasiliensis fruit bodies |
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| 中文关键词:巴西蘑菇，硒，镧，子实体，孢子，囊状体 |
| 英文关键词:Agaricus brasiliensis, Selenium, Lanthanum, fruit body, spore, cystidia |
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| 中文摘要: |
| 采用混合培养料试验和电子显微技术，观察了不同硒镧配施水平对巴西蘑菇子实体盖皮和菌褶表面形态及孢子和囊状体数量特征的影响。结果表明，低浓度硒镧配施（Na2SeO3 21.905mg/kg + LaCl3 17.667mg/kg）处理时，孢子数极显著增加，随着硒镧配施浓度升高，孢子数极显著增加，达到峰值后极显著减少；低浓度（Na2SeO3 21.905mg/kg + LaCl3 17.667mg/kg）和高浓度（Na2SeO3 109.524mg/kg + LaCl3 88.340mg/kg）处理后囊状体数极显著减少，中浓度（Na2SeO3 65.714mg/kg + LaCl3 53.002mg/kg）处理囊状体数显著增加。研究还发现硒镧配施对巴西蘑菇孢子长、宽、囊状体最大直径和最小直径以及菌盖皮菌丝最大宽度具有促进或抑制作用。扫描电镜观察结果表明，与对照相比，中浓度硒镧配施（Na2SeO3 65.714mg/kg + LaCl3 53.002mg/kg）时，孢子由正常椭圆形变成全部萎缩，菌丝粗壮；囊状体在较低硒镧配施浓度（Na2SeO3 43.810mg/kg + LaCl3 35.335mg/kg）处理时表面纹饰模糊、消失，个别囊状体破裂，表面带有藤蔓状的组织。 |
| 英文摘要: |
| The multiple effects of Se and La on morphological and quantitative characters of Agaricus brasiliensis fruit bodies were studied by cultivation of the fungus on medium mixed with different concentration of solutions of Na2SeO3 and LaCl3 and electron microscopy technology. The results showed that there was a very significant increase in sporulation under the treatment of low concentration of Na2SeO3 21.905mg/kg + LaCl3 17.667mg/kg. The number of spores increased with the increase of concentration of the mixed solution and significantly decreased after reaching the peak. The number of cystidia significantly reduced when the fungus grew in the medium with low concentrations (Na2SeO3 21.905mg/kg+LaCl3 17.667mg/kg) and high concentration (Na2SeO3 109.524mg/kg+LaCl3 88.340mg/kg) of Se and La, but the number of cystidia significantly increased under the moderate concentration (Na2SeO3 65.714mg/kg+LaCl3 53.002mg/kg). It was found that the combined application of Se and La affected the length and width of spore, the diameters of cystidia and the width of mycelium. Scanning electron microscopy showed that the spores were shrinking and collapsing, and the mycelium walls were ramified and nodulated under combined application of Na2SeO3 65.714mg/kg+LaCl3 53.002mg/kg. Furthermore the surface ornamentation of cystidia became fuzzy and disappearing, and some cystidia were disappearing and collapsing. |
| 江枝和* 翁伯琦 王义祥 雷锦桂. 硒镧复合作用对巴西蘑菇子实体形态和数量特征的影响[J]. 菌物学报, 2008, 27(5): 679-687 |
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