| β-蜕皮激素对蛹虫草液体培养菌丝体生长及代谢产物的促进作用 |
| β-ecdysone increases the growth and metabolites of Codyceps militaris mycelia in submerged culture |
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| 中文关键词: |
| 英文关键词:extracellular polysaccharide, intracellular polysaccharide, cordycepin, secretion volume, fermentation |
| 基金项目: |
| 作者 | 单位 | | 施明珠 | Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Key Laboratory of Microbial Control of Anhui Province, Anhui Agricultural University, Hefei 230036, China
| | 陶苏丹 | College of Life Sciences, Nankai University, Tianjin 300071, China | | 钟 石 | Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | 李有贵 | Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | 李增智 | Key Laboratory of Microbial Control of Anhui Province, Anhui Agricultural University, Hefei 230036, China | | 计东风 | Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| 摘要点击次数: 2001 |
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| 中文摘要: |
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| 英文摘要: |
| The effects of β-ecdysone on mycelial dry weight (MDW) and the yield of extracellular polysaccharide (EPS), intracellular polysaccharide (IPS) and cordycepin of Codyceps militaris in submerged cultivation was investigated. Adding 12mg/L of β-ecdysone to the culture medium, the MDW, the secretion volume of EPS per unit of MDW and total yield of EPS were increased by 18.5%, 181.7% and 63.2%, respectively. The yield of IPS was enhanced by 29.2%, when 1.2mg/L of β-ecdysone was added. When 4mg/L of β-ecdysone was provided, the amount of cordycepin per unit of MDW and the total yield of cordycepin were increased by 65.4% and 84.7%, respectively. These results demonstrated that β-ecdysone promoted the yield of MDW, EPS, IPS and cordycepin of Codyceps militaris mycelia, though this effect varied among different components. Significantly, β-ecdysone showed a more positive role in secondary metabolite (cordycepin) accumulation than in primary metabolite (IPS) production and growth of mycelia. Furthermore, β-ecdysone stimulated the secretion of EPS. |
| 施明珠,陶苏丹,钟 石,李有贵,李增智,计东风. β-蜕皮激素对蛹虫草液体培养菌丝体生长及代谢产物的促进作用[J]. 菌物学报, 2008, 27(4): 559-566 |
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