| 蝉拟青霉代谢产物清除DPPH自由基和抗真菌活性的研究 |
| Scavenging and anti-fungal activities of the metabolite of Paecilomyces cicadae |
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| 中文关键词: |
| 英文关键词:entomogenous fungi, mycelia, fermentation broth, biological activities |
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| 英文摘要: |
| DPPH-TLC and DPPH-Microplate assays were used to determine the free radical scavenging activities of methanol extracts from the fermentation broth and the mycelia of Paecilomyces cicadae P3. The results revealed that the extracts had strong free radical scavenging activities. At the concentration of 5.0mg/mL the methanol extract from fermentation broth of P3 could decrease 74.86% of 0.4mg/mL DPPH radicals after incubating at 37℃ for 10 minutes. An anti-fungal TLC assay was applied to test antimicrobial metabolites of Paecilomyces cicadae P3. Meanwhile, an anti-Candida albicans Oxford Cup assay was made to confirm their anti-fungal activities. At the concentration of 5.0mg/mL the methanol extracts from fermentation broth and mycelia of P3 had significant anti-Candida albicans activities and the diameters of inhibition zone were 21.42mm and 11.23mm respectively. |
| 陈安徽，李春如，樊美珍. 蝉拟青霉代谢产物清除DPPH自由基和抗真菌活性的研究[J]. 菌物学报, 2008, 27(3): 405-412 |
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