台湾虫草及其无性型关系的分子确证 |
Molecular identification for connection of Cordyceps formosana and its Hirsutella anamorph |
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中文关键词:黄山被毛孢,rDNA-ITS 区,虫生真菌 |
英文关键词:Hirsutela huangshanensis, rDNA-ITS, entomopathogenic fungi |
基金项目:国家自然科学基金(No. 30570004);国家高技术研究发展计划资助项目(863计划)(No. 2007AA021506);安徽省自然科学基金(No. 070411011);安徽高校省级自然科学研究重点项目(No. TD200708) |
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中文摘要: |
采用PCR技术,以rDNA-ITS区为分子指标,对台湾虫草Cordyceps formosana及其无性型黄山被毛孢Hirsutella huangshanensis进行测序和比较分析,以进一步确定两者的对应关系。结果表明:两者的序列完全相同,从而在分子水平上证明了黄山被毛孢是台湾虫草的无性阶段。系统进化树显示台湾虫草的无性型归为被毛孢属较为合理。 |
英文摘要: |
The genes of the 5.8S rRNA and the complete internal transcribed spacer (ITS) regions from Cordyceps formosana and Hirsutella huangshanensis were sequenced and compared. The results showed that Cordyceps formosana and Hirsutella huangshanensis had the same ITS1-5.8S-ITS2 nucleotide sequences, strongly supporting the fact that Cordyceps formosana was the teleomorph of Hirsutella huangshanensis. Molecular phylogenetic analysis demonstrated that the conidial state of C. formosana belonged to the genus Hirsutella. |
左登平,李春如,黄勃,何亚琼,樊美珍,李增智. 台湾虫草及其无性型关系的分子确证[J]. 菌物学报, 2008, 27(2): 224-229 |
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