| 不同培养基和三种金属离子对桦褐孔菌培养菌丝体的羊毛甾醇和麦角甾醇积累的影响 |
| Effects of culture media and three metal ions on the accumulation of lanosterol and ergosterol in cultured mycelia of Inonotus obliquus |
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| 中文关键词: |
| 英文关键词:medical fungus, carbon source, nitrogen source, treatment of metal ions, sterol accumulation, HMG-CoA reductase activity |
| 基金项目: |
| 作者 | 单位 | | 郑维发 | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China | | 项晓燕 | School of Biological Sciences, Anqing Normal College, Anqing 26400, China | | 陈才法 | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China | | 王尧 | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China | | 赵艳霞 | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China | | 蒋继宏 | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China | | 储成才 | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
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| 摘要点击次数: 1891 |
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| 中文摘要: |
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| 英文摘要: |
| Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4?7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35?mol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I. obliquus. |
| 郑维发,项晓燕,陈才法,王尧,赵艳霞,蒋继宏,储成才. 不同培养基和三种金属离子对桦褐孔菌培养菌丝体的羊毛甾醇和麦角甾醇积累的影响[J]. 菌物学报, 2008, 27(1): 126-139 |
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