富铬蚁巢伞(鸡 菌)液体培养 |
Submerged culture of chromium-enriched Termitomy-ces albuminosus |
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中文关键词: |
英文关键词:glucose tolerance factor, medium optimization, growth kinetics |
基金项目: |
作者 | 单位 | 胡忠策 | College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China | 郑晓冬 | College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China | 陈新爱 | College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China | 郑裕国 | Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China |
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中文摘要: |
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英文摘要: |
A species of mushroom, Termitomyces albuminosus, was cultured in liquid medium for production of chromium-enriched mycelium. The influence of chromium (Ⅲ) on mycelial growth of T. albuminosus was investigated. An optimum medium composed of 5.6g/L yeast extract, 51.6g/L hydrolyzed rice, 2g/L KH2PO4, and 20mg/L chromium(Ⅲ) with initial pH of 4.5 was obtained by using method of central composite design (CCD). After incubation of 84h, the maximal biomass of chromium-enriched mycelia reached 24.23g DMW(dried mycelial weight)/L with 272μg/g DMW chromium content in 500mL flasks containing 100mL medium with an inoculum of 8% on a shaker of 100r/min under an optimized cultivation condition at 28℃. |
胡忠策,郑晓冬,陈新爱,郑裕国. 富铬蚁巢伞(鸡 菌)液体培养[J]. 菌物学报, 2008, 27(1): 118-125 |
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