| 编码腈水解酶的大豆疫霉基因PsNIA的克隆与转录分析 |
| Cloning and transcription of PsNIA, an encoding putativenitrilase gene from the oomycete plant pathogen Phytophthora sojae |
| 投稿时间：2007-04-06 修订日期：2007-05-31 |
| 中文关键词:大豆疫霉，腈水解酶，PsNIA，致病性 |
| 英文关键词:Phytophthora sojae, nitrilase, PsNIA, pathogenicity |
| 基金项目:国家自然科学基金资助项目（No. 30471124） |
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| 中文摘要: |
| 包括大豆在内的许多植物都可以产生氰化物，对侵染的病原菌产生毒害作用而阻碍其进一步扩展。采用抑制性差减杂交(suppression subtractive hybridization，SSH)的方法，筛选到一个在大豆疫霉侵染早期上调表达的、编码腈水解酶的cDNA片段；克隆了该基因的全长序列，命名为PsNIA。Southern杂交结果显示，PsNIA在大豆疫霉基因组中只有1个拷贝。系统发育分析表明，PsNIA与绿脓杆菌Pseudomonas aeruginosa的腈水解酶的序列同源性最高，且该基因编码的氨基酸序列具有腈水解酶的保守结构域。RT-PCR分析表明，该基因在大豆疫霉侵染大豆12h时可以检测到转录。 |
| 英文摘要: |
| The release of hydrogen cyanide (HCN) from preformed cyanogenic compounds in plants such as soybean is thought to provide a protective barrier against infection by microorganisms. Using the method of suppression subtractive hybridization (SSH), a Phytophthora sojae cDNA coding nitrilase was identified, which was up-regulated during the early stage of soybean infection. The termed PsNIA gene possessed one copy in the P. sojae genome. Its amino acid sequence harbored overall conserved domain of nitrilase, homologous to nitrilase of Pseudomonas aeruginosa. The transcriptional level of PsNIA was up-regulated throughout infection. |
| 王子迎. 编码腈水解酶的大豆疫霉基因PsNIA的克隆与转录分析[J]. 菌物学报, 2007, 26(4): 549-556 |
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