| 马铃薯晚疫病菌DK98-1、DX98-2和DX98-3的生物学特性及AFLP指纹分析 |
| Biological characteristics and AFLP fingerprints of Phytophthora infestans strains DK98-1, DX98-2 and DX98-3 |
| 投稿时间：2006-11-10 修订日期：2007-03-10 |
| 中文关键词:致病疫霉，卵孢子，特异菌株，专一性引物 |
| 英文关键词:Phytophthora infestans, oospore, specific strains, specific primers |
| 基金项目:河北省自然科学基金（No.C2004000379）；河北农业大学重点科技项目（No.9816） |
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| 中文摘要: |
| 利用黑麦培养基和V8-蔬菜汁培养基研究了马铃薯晚疫病菌Phytophthora infestans特异菌株DK98-1、DX98-2和DX98-3 的生物学特性，发现该菌株与普通菌株相比菌落生长速度慢、孢子囊产生数量少、有性杂交后卵孢子产生量大（2047～75623个/cm2）；利用AFLP分子标记研究这3个菌株的DNA指纹图谱，发现用引物E+CG/M+CC扩增菌株DK98-1、DX98-2和DX98-3后，在330bp处与普通菌株相比各缺失一条谱带，用引物E+AC/M+CT扩增菌株DK98-1、DX98-2和DX98-3后，在370bp处比普通菌株增加1条谱带，说明这3个菌株与普通菌株在遗传上明显不同。同时可以利用上述2对特异性引物，鉴定在自然界的晚疫病菌群体中这类特异菌株的出现频率。 |
| 英文摘要: |
| Biological characteristics of the Phytophthora infestans strains DK98-1, DX98-2 and DX98-3 have been studied on the rye medium and V8-vegetable juice medium. As compared with the common strains, the colony growth of these strains was slow, the quantity of sporangia was few, and the quantity of oospore(2047～75623/cm2) produced was much more after sexual hybridization. DNA fingerprints of the 3 strains were detected by AFLP molecular marker. Strains DK98-1, DX98-2 and DX98-3 lack one band as compared with common strains on site of 330bp after they were amplified with the primer E+CG/M+CC, while they increase one band on site of 370bp after they were amplified with the primer E+AC/M+CT. It is indicated that these three strains distinguish from common strains at the genetic background. The frequency of the specific P. infestans strains could be investigated in nature by using these 2 specific primers. |
| 朱杰华,杨志辉,刘大群,刘顺. 马铃薯晚疫病菌DK98-1、DX98-2和DX98-3的生物学特性及AFLP指纹分析[J]. 菌物学报, 2007, 26(3): 455-462 |
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