| 不同培养条件对中国被毛孢胞内核苷类组分的影响 |
| Effects of different culture condition on nucleoside content in the mycelia of Hirsutella sinensis |
| 投稿时间：2006-09-12 修订日期：2006-12-21 |
| 中文关键词:冬虫夏草 虫生真菌 活性成分 培养基配方 液体培养 |
| 英文关键词:Cordyceps sinensis entomogenous fungi bioactive component media components liquid culture |
| 基金项目:国家自然科学基金资助项目(No.30570189和30570004) |
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| 中文摘要: |
| 采用紫外分光光度法,对冬虫夏草Cordyceps sinensis无性型——中国被毛孢Hirsutella sinensis液体摇瓶培养时胞内核苷类组分的形成积累条件进行了研究。结果表明:菌丝体中核苷类成分形成积累的最适摇瓶培养条件是碳源为葡萄糖:氮源为酵母浸出粉:微量元素为MgSO4·7H2O和KH2PO4;起始pH值6．5:摇瓶装液量100mL/500mL:摇床转数180r/min:接种量10％。正交试验结果表明最佳培养基配方为葡萄糖2％,酵母浸出粉3％,奶粉1％,MgSO4·7H2O 0．1％,KH2PO40．2％。培养条件优化后,菌丝体中总核苷的含量可达6．5748mg/g,比优化前提高了33．6％。 |
| 英文摘要: |
| The optimum medium and optimal cultivation conditions of Hirsutella sinensis were studied,and the accumulation of nucleosides in the mycelia of shake culture were investigated by ultraviolet spectrophotometry. The results showed that the formula of appropriated culture medium was as follows: glucose 2%,yeast powder 3%,milk powder 1%,MgSO4.7H2O 0.1% and KH2PO4 0.2%.The suitable culture conditions were as follows: pH 6.5,medium volume 100mL/500mL,rotation rate 180r/rain,inoculum amount 10%.The total nucleoside content reached 6.5748mg/g in the mycelia fermented under optimized culture condition,improved by 33.6% as compared with that under initial culture condition. |
| 葛飞,李春如,胡丰林,桂琳,周权,陈安徽,樊美珍. 不同培养条件对中国被毛孢胞内核苷类组分的影响[J]. 菌物学报, 2007, 26(2): 234-242 |
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