| 云南野生大白口蘑遗传多样性研究 |
| Genetic diversity of Tricholoma giganteum Massee in Yunnan |
| 投稿时间：2006-03-14 修订日期：2006-08-08 |
| 中文关键词:菌塘 直接扩增片断长度多态性 |
| 英文关键词:Shiro Direct amplification of length polymorphism （DALP） |
| 基金项目:贵州省教育厅自然科学基金资助项目 (No.2004217) |
|
| 摘要点击次数: 1394 |
| 全文下载次数: 458 |
| 中文摘要: |
| 应用DALP技术对云南野生大白口蘑遗传多样性进行分析。筛选的5组引物在大白口蘑6个菌塘的56朵子实体中检测到202个位点,其中多态位点151个,多态位点百分比PPB为74.75%。6个菌塘总的观察等位基因数Na为1.7475;总的有效等位基因数Ne为1.4659;Nei’s基因多样性指数H为0.2722;总的Shannon多样性指数I为0.4053;菌塘总的基因多样度Ht为0.2705,菌塘内基因多样性度Hs为0.012,菌塘间基因分化系数Gst为0.9556,菌塘的遗传一致度在0.60~0.80之间。结果表明大白口蘑遗传变异有95.56%存在菌塘间,4.44%存在菌塘内,即大白口蘑遗传变异绝大多数存在于菌塘之间。 |
| 英文摘要: |
| Genetic diversity of Tricholoma giganteum in Yunnan was determined by using DALP analysis. Five primer groups were screened in 6 shiroes of T. giganteum. Among 202 DNA fragments amplified 151 were polymorphic, the percentage of polymorphic bands (PPB) was 74.75%. In the 6 shiroes, the total observed number of alleles (Na) was 1.7475; the total effective number of alleles (Ne) was 1.4659; the total Nei’s gene diversity (H) was 0.2722; the total Shannon's Information index (I) was 0.4053; the total gene diversity (Ht)was 0.2705 while the gene diversity within a shiro (Hs)was 0.012.The coefficient of gene differentiation (Gst)was 0.9556 among shiroes,Nei's genetic identity was at the range of 0.60~0.80.The result indicated that 95.56% genetic variation occurred among shiroes and 4.44% within a shiro,it was suggested that most of genetic variation be among shiroes of T.giganteum. |
| 汤洪敏,吴刚,虞泓. 云南野生大白口蘑遗传多样性研究[J]. 菌物学报, 2007, 26(1): 128-134 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|