| 应用RAPD标记对斜生褐孔菌菌株亲缘关系的分析 |
| RAPD analysis of Phaeoporus obliquus strains from different areas of China |
| 投稿时间：2006-03-20 修订日期：2006-09-10 |
| 中文关键词:菌核 DNA指纹图谱 遗传距离 |
| 英文关键词:Sclerotium DNA fingerprint genetic distance |
| 基金项目:国家自然科学基金资助项目部分内容 (30560093) |
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| 中文摘要: |
| 采用RAPD技术对采集不同地区的8个斜生褐孔菌野生菌核分离得到的菌株亲缘关系进行研究,获得了斜生褐孔菌不同菌株的DNA指纹图谱。结果显示:12个引物共扩增出167条带,其中101条为多态性带,多态性比率为60.5%,同一培养时期的各菌株间RAPD图谱表明菌株间存在一定的种内及地理来源差异。若以遗传距离0.508为结合线,可将供试菌株划分为三大类,BCX01、BCX02归为一类,JL01、JL02、JL03、JL04、JL05归为一类,HLJ01单独聚为一类。 |
| 英文摘要: |
| The relationship of 8 Phaeoporus obliquus strains isolated from sclerotia collected from different areas of China was studied by use of random amplified polymorphic DNA (RAPD) markers. The DNA fingerprints of different strains showed that there were genetic diversity among the strains tested. The results indicated that 12 random primers generated 167 bands in total,of which 101 bands showed polymorphism and the average polymorphism rate was 60.5%. There are a little intraspecific difference among fingerprints of the strains.At the level of 0.508 (genetic distance),BCX01 and BCX02 were clustered in the same group and the second group included JL01,JL02,JL03,JL04 and JL05.HLJ01 was independent.Genetic variability of different strains is related to geographical origins. |
| 陈艳秋,李玉,郭晓帆. 应用RAPD标记对斜生褐孔菌菌株亲缘关系的分析[J]. 菌物学报, 2007, 26(1): 122-127 |
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