| 60Co辐射诱变对巴氏蘑菇(姬松茸)子实体多糖组分与结构变化的影响 |
| Effects of 60Co irradiation on the components and structure of polysaccharide from the fruitbodies of Agaricus blazei Murrill |
| 投稿时间：2006-07-18 修订日期：2006-09-20 |
| 中文关键词:巴氏蘑菇 子实体 多糖 结构分析 |
| 英文关键词:Agaricus blazei fruitbodies polysaccharide structure analysis |
| 基金项目:福建省科技厅科技项目(2003N047，2005N029) |
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| 中文摘要: |
| 巴氏蘑菇(姬松茸)原菌株J1和辐射突变株J3子实体粗多糖经水提、醇沉、Sevage法去除蛋白,透析得精多糖ABJ-1和ABJ-3;经紫外扫描和凝胶色谱法检测表明,ABJ-1和ABJ-3均具有较高的纯度。以苯酚-硫酸法测得了ABJ-1和ABJ-3的糖含量分别为47%和76.68%,凯氏定氮法测定其含氮量分别为5.77%和21%。,以凝胶过滤法测定了其分子量均在4万左右。完全酸水解后气相色谱分析显示ABJ-1含有甘露糖、葡萄糖、半乳糖等单糖,ABJ-3含有大量的甘露糖及少量的葡萄糖等单糖。红外光谱和Smith降解后气相色谱分析显示ABJ-1和ABJ-3糖基连接键均以a,1-4糖苷键为主。 |
| 英文摘要: |
| Polysaccharides from the fruitbodies of Agaricus blazei J1and J3 were extracted and the polysaccharide structure was analyzed. Purified ABJ-1 and ABJ-3 were obtained from crude polysaccharides of Agaricus blazei by water-extracting, alcohol-sedimentation, protein-cleaning(Sevage method) and polysaccharide purification(dialysis method). Their homogeneity were verified by UV scan and gel chromatography. The carbohydrate content of ABJ-1 and ABJ-3 were 47% and 76.68%, respectively. Their nitrogen contents were 5.77%and 21%,respectively,Their molecular weight were around 40000.The results of gas phase chromatographic analysis after complete acid hydrolysis showed that the composition of ABJ-1 was mannose,glucose,galactose and so on,and the composition of ABJ-3 was mannose and a few glucose.The results of IR spectrum analysis and Smith degradation showed that their glycosyl linkage were mostly α,1-4. |
| 翁伯琦,江枝和,宾文,王义祥,罗涛,林勇. 60Co辐射诱变对巴氏蘑菇(姬松茸)子实体多糖组分与结构变化的影响[J]. 菌物学报, 2007, 26(1): 106-114 |
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