| 应用简并引物扩增黑木耳信息素受体基因片段 |
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| 中文关键词:裂褶菌 交配型因子 跨膜结构域 连锁 |
| 英文关键词: |
| 基金项目: |
| 肖扬 唐利华 边银丙* |
| 华中农业大学应用真菌研究所 |
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| 中文摘要: |
| Degenerate primers, br1-F and br1-R, designed based on the conserved amino acid sequence of STE3 pheromone receptor in Schizophyllum commune, were used to amplify genomic DNA of monkaryotic parental strains(H2, J3) and fifty-nine monokaryons of their F1 progenies in Auricularia auricula. A fragment of the PCR product 811bp in length were amplified from the parental strain H2, nine monokaryons of the H2 mating –type and fifteen ones of the J3 mating –type of F1 progenies. After cloning , sequencing the fragm… |
| 英文摘要: |
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| 肖扬,唐利华,边银丙. 应用简并引物扩增黑木耳信息素受体基因片段[J]. 菌物学报, 2006, 25(2): |
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