| 赤霉菌原生质体DNA转化* |
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| 中文关键词:赤霉菌 原生质体 DNA转化 潮霉素B抗性基因 |
| 英文关键词: |
| 基金项目: |
| 朱平** $2 朱慧新 程克棣** |
| 中国医学科学院 中国协和医科大学 药物研究所卫生部天然药物生物合成重点实验室 |
| 摘要点击次数: 1840 |
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| 中文摘要: |
| Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene ha… |
| 英文摘要: |
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| 朱平,$2,朱慧新,程克棣. 赤霉菌原生质体DNA转化*[J]. 菌物学报, 2000, 19(2): |
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