大肠杆菌利用葡萄糖和木糖合成乙醇酸、乳酸和3-羟基丁酸共聚酯
Microbial production of poly (glycolate-co-lactate-co-3- hydroxybutyrate) from glucose and xylose by Escherichia coli
投稿时间:2018-05-11  
DOI:  10.13345/j.cjb.180199
中文关键词:聚羟基脂肪酸酯,乙醇酸,乳酸,3-羟基丁酸,葡萄糖,木糖
英文关键词:polyhydroxyalkanoate, glycolate, lactate, 3-hydroxybutyrate, glucose, xylose
基金项目:国家自然科学基金 (No. 21476014),国家科技支撑计划 (No. 2015BAD15B09) 资助。
作者单位E-mail
笪央央 北京化工大学 生命科学与技术学院北京 100029  
李微 北京化工大学 生命科学与技术学院北京 100029  
史理陇 北京化工大学 生命科学与技术学院北京 100029  
李正军 北京化工大学 生命科学与技术学院北京 100029 lizj@mail.buct.edu.cn 
摘要点击次数: 288
全文下载次数: 312
中文摘要:
      以大肠杆菌为宿主,构建了以葡萄糖和木糖为底物获得乙醇酸、乳酸和3-羟基丁酸共聚酯的生物合成途径,包括过表达塔格糖-3-差向异构酶、核酮糖激酶、醛缩酶、醛脱氢酶、丙酰辅酶A转移酶、β-酮硫解酶、乙酰乙酰辅酶A还原酶和聚合酶等。在此基础上,表达聚羟基脂肪酸酯颗粒结合蛋白,提高了聚合物的合成,重组菌的细胞干重达到3.73 g/L,含有38.72 wt%的共聚酯。采用混菌共培养策略,实现以葡萄糖和木糖混合物为底物合成共聚酯,摇瓶实验中细胞干重达到4.01 g/L,含有21.54 wt%的聚合物。文中提供了一种以葡萄糖和木糖混合物为碳源合成聚合物的方法,为下一步纤维素水解物的有效利用提供了参考。
英文摘要:
      Escherichia coli was metabolically engineered to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate) using glucose and xylose as carbon sources. The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase. Overexpression of polyhydroxyalkanoate granule associated protein significantly improved biopolymer synthesis, and the recombinant strain reached 3.73 g/L cell dry weight with 38.72% (W/W) biopolymer content. A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54% (W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.
笪央央,李微,史理陇,李正军.大肠杆菌利用葡萄糖和木糖合成乙醇酸、乳酸和3-羟基丁酸共聚酯[J].生物工程学报,2019,35(2):254~262
HTML   查看全文   查看/发表评论  下载PDF阅读器