液滴微流控芯片系统中微液滴特性表征及氨基酸检测应用
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中国科学院科研装备研制项目(No. YZ201153),国家自然科学基金(Nos. 21206162, 21205134)资助。


Micro-droplet characterization and its application for amino acid detection in droplet microfluidic system
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Instrument Developing Project of the Chinese Academy of Sciences (No. YZ201153), National Natural Science Foundation of China (Nos. 21206162, 21205134).

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    摘要:

    基于液滴微流控芯片的检测和筛选系统,因其具有通量高、成本低等显著特点,近年来备受关注。该系统生成的微液滴 (皮升体积) 具有直径均一、大小可控、互不交融 (单分散性) 等特性,它作为微反应器可以进行微生物及其代谢物包埋实验和高通量检测。因此,研究微液滴的相关特性及其应用具有重要意义。文中在液滴微流控芯片系统搭建的基础上,对重要氨基酸 (谷氨酸、苯丙氨酸、色氨酸和酪氨酸) 分别进行了微液滴包埋实验,研究了包埋微液滴的重要特性参数 (如稳定性、扩散性等) ,探索了包埋微液滴对氨基酸检测分选的应用。实验表明,文中搭建的液滴微流控芯片系统可以稳定、均一地生成微液滴,微液滴大小可根据需要控制在20?50 μm之间,微液滴间无交叉污染,包埋氨基酸的微液滴的检测筛选速度大约为每分钟600个。这个研究为高通量分析和筛选产氨基酸的微生物奠定了基础。

    Abstract:

    Recently, the droplet microfluidic system attracts interests due to its high throughput and low cost to detect and screen. The picoliter micro-droplets from droplet microfluidics are uniform with respect to the size and shape, and could be used as monodispensed micro-reactors for encapsulation and detection of single cell or its metabolites. Therefore, it is indispensable to characterize micro-droplet and its application from droplet microfluidic system. We first constructed the custom-designed droplet microfluidic system for generating micro-droplets, and then used the micro-droplets to encapsulate important amino acids such as glutamic acid, phenylalanine, tryptophan or tyrosine to test the droplets’ properties, including the stability, diffusivity and bio-compatibility for investigating its application for amino acid detection and sorting. The custom-designed droplet microfluidic system could generate the uniformed micro-droplets with a controllable size between 20 to 50 μm. The micro-droplets could be stable for more than 20 h without cross-contamination or fusion each other. The throughput of detection and sorting of the system is about 600 micro-droplets per minute. This study provides a high-throughput platform for the analysis and screening of amino acid-producing microorganisms.

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袁会领,董立兵,涂然,杜文斌,贾士儒,王钦宏. 液滴微流控芯片系统中微液滴特性表征及氨基酸检测应用[J]. 生物工程学报, 2014, 30(1): 139-146

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  • 收稿日期:2013-07-20
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  • 在线发布日期: 2014-01-07
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