发酵产丁二酸过程中废弃细胞的循环利用
Recycle of spent cells from anaerobic succinate fermentation
投稿时间:2010-03-01  
DOI:  
中文关键词:废弃细胞,酶水解,氮源,丁二酸
英文关键词:recycle spent cells, enzymatic hydrolysis, nitrogen source, succinic acid
基金项目:国家重点基础研究发展计划 (973计划) (No. 2009CB724701),国家自然科学基金 (No. 20606017),材料化学工程国家重点实验室基金,江苏省“青蓝工程”资助。
作者单位E-mail
白雪飞 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009  
陈可泉 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009  
叶贵子 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009  
黄秀梅 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009  
李建 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009  
姜岷 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室南京 210009 jiangmin@njut.edu.cn 
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中文摘要:
      对厌氧发酵产丁二酸后的废弃细胞进行破壁处理,考察了以细胞水解液作为有机氮源重新用于丁二酸发酵的可行性。比较了超声破碎、盐溶、酶解3种方法破碎细胞获得的水解液作为氮源发酵产丁二酸的效果,结果表明酶解制得的细胞水解液效果最佳。以总氮含量为1.11 g/L的酶解液 (相当于10 g/L酵母膏) 作为氮源发酵,丁二酸产量可达42.0 g/L,继续增大酶解液用量对耗糖、产酸能力没有显著提高。将细胞酶解液与5 g/L酵母膏联用发酵36 h后,丁二酸产量达75.5 g/L,且丁二酸生产强度为2.10 g/(L·h),比
英文摘要:
      Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L (equivalent to 10 g/L yeast extract), the succinic acid concentration was 42.0 g/L, but it increased slightly when enhancing the level of enzymatic hydrolysate. However, when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells, the succinic acid concentration reached 75.5 g/L after 36 hours and, the succinic acid productivity was 2.10 g/(L·h), which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore, enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.
白雪飞,陈可泉,叶贵子,黄秀梅,李建,姜岷.发酵产丁二酸过程中废弃细胞的循环利用[J].生物工程学报,2010,26(9):1276~1280
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