菌株DN2对烟草薄片制备液中烟碱的降解
Biodegradation of nicotine in tobacco extracts for making reconstituted tobacco by strain DN2
投稿时间:2009-02-01  
DOI:  
中文关键词:烟草薄片, O. intermedium DN2, 烟碱, 生物降解
英文关键词:reconstituted tobacco, O. intermedium DN2, nicotine, biodegradation
基金项目:
作者单位E-mail
袁勇军 浙江万里学院生物与环境学院, 宁波 315100
南京农业大学食品科技学院, 南京 210095 
 
陆兆新 南京农业大学食品科技学院, 南京 210095 fmb@njau.edu.cn 
戚向阳 浙江万里学院生物与环境学院, 宁波 315100  
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中文摘要:
      使用O. intermedium DN2降解烟草薄片制备液中的烟碱。研究了各种工艺参数对烟碱降解的影响, 单因素考察结果表明烟草薄片制备液中烟碱降解的最适条件是: 添加0.1%的酵母膏, 使用氨水将pH调节到7.0, 接种15%种子液, 培养温度30oC。在上述条件下, 采用30L发酵罐对烟草薄片制备液进行3个批次的半连续发酵, 烟碱的平均降解速率为140.55 mg/L/h, 高于其他烟碱降解菌株。该结果表明菌株DN2可以用来降低烟草薄片中的烟碱含量。
英文摘要:
      The purpose of the study is to use O. intermedium DN2 to degrade nicotine in tobacco extracts for making reconstituted tobacco. Firstly, we studied the effects of various factors on degradation of nicotine in the extracts by strain DN2. When we added 0.1% yeast extract into the extracts, adjusted its pH value to 7.0 by ammonia solution, inoculated 15% cultures and maintained fermentation temperature of 30°C, the degradation rate of nicotine by strain DN2 was the fastest. Furthmore, under these conditions, we studied the degradation rates of nicotine in three fed batches culture which carried out in a 30-L reactor, the result showed that the average degradation rate of nicotine by strain DN2 was 140.55 mg/L/h, which was much higher than that reported in other studies. These results indicated that strain DN2 may be useful for reducing nicotine content of reconstituted tobacco.
袁勇军,陆兆新,戚向阳.菌株DN2对烟草薄片制备液中烟碱的降解[J].生物工程学报,2009,25(6):897~902
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