猪I型与II型干扰素的克隆、表达及抗病毒活性比较
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家科技支撑计划(No. 2006BAD06A04), 中国科学院百人计划资助。


Comparison of antiviral activities of porcine interferon type I and type II
Author:
Affiliation:

Fund Project:

Ministry of Science and Technology of China Program (No. 2006BAD06A04), Hundreds of Talents Program of Chinese Academy of Sciences.

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    干扰素(IFN)是由多种细胞受病毒感染或其他生物诱导剂刺激而产生的天然蛋白质, 主要功能为抗病毒增殖、调节免疫反应和激活免疫细胞等。本研究克隆并测序了猪干扰素(PoIFN)a、g、a g及w基因。构建原核表达载体pET-His/PoIFN-a、pET-His/PoIFN-g、pET-His/PoIFN- ag和pET-His/PoIFN-w, 转化大肠杆菌Rosetta(DE3)进行表达, 经纯化、复性得到具有生物学活性的蛋白。用细胞病变抑制法在Marc-145/PRRSV、Marc-145/VSV、PK-15/VSV、Vero/VSV、MDBK/VSV系统上进行抗病毒活性测定, 结果表明猪a和ag融合干扰素有较为显著的抗病毒活性, 抗PRRSV活性高达108 U/mg; 猪g干扰素活性效价约为a干扰素的1/2到1/3; 猪w干扰素几乎未检测到抗病毒活性, 需进一步验证。本研究对干扰素在抗病毒、提高机体免疫方面的应用提供了理论依据。

    Abstract:

    Interferons (IFNs) are natural proteins produced by wide variety of cells in response to viral infection or other biological inducers, and they execute diversified functions as antiviral defense, immune activation and cell growth regulation. Four genes encoding porcine interferons (PoIFN), PoIFN-α, PoIFN-γ, PoIFN-αγ or PoIFN-ω, were cloned and sequenced. The four types of porcine interferon genes were subcloned into the pET-His vector, and expressed in Escherichia coli Rosetta (DE3). The recombinant products were purified and renaturalized from inclusion bodies to obtain a native state of well biological activity. Antiviral activity assays for porcine interferons were performed and evaluated by standard procedures in following cell/virus test systems: Marc-145/PRRSV, Marc-145/VSV, PK-15/VSV, Vero/VSV or MDBK/VSV. The data showed that both PoIFN-α and PoIFN-αγ demonstrated significant antiviral activities, and the titer of them against PRRSV was up to 108 U/mg. PoIFN-γ had approximately half or one-thirds antiviral activity of PoIFN-α. PoIFN-ω showed inconspicuous antiviral activity.

    参考文献
    相似文献
    引证文献
引用本文

陈雪梅,薛清华,祝荣格,付显华,杨利敏,孙蕾,刘文军. 猪I型与II型干扰素的克隆、表达及抗病毒活性比较[J]. 生物工程学报, 2009, 25(6): 806-812

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-03-18
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
您是第位访问者
生物工程学报 ® 2024 版权所有

通信地址:中国科学院微生物研究所    邮编:100101

电话:010-64807509   E-mail:cjb@im.ac.cn

技术支持:北京勤云科技发展有限公司