微生物菌种改良的新方法新策略
Novel methods and strategies for strain improvement
投稿时间:2008-12-10  
DOI:  
中文关键词:菌种改良, 抗生素生物合成, 代谢工程, 基因组改组, 系统生物技术, 核糖体工程, 表观遗传修饰
英文关键词:strain improvement, antibiotic biosynthesis, metabolic engineering, genome shuffling, system biotechnology, ribosome engineering, epigenetic modification
基金项目:重庆市科技攻关重点项目(No. CSTC 2009AB1029)资助。
作者单位E-mail
赵文婷 西南大学药学院 现代生物医药研究所, 重庆 400716  
邹懿 西南大学药学院 现代生物医药研究所, 重庆 400716  
胡昌华 西南大学药学院 现代生物医药研究所, 重庆 400716 chhhu@swu.edu.cn 
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中文摘要:
      自然分离得到的原始菌种远不能达到工业生产要求, 通过菌种改良获得高产菌种是有效的手段。传统方法虽然无需了解过多遗传背景就能取得成效, 但往往耗时费力。随着DNA重组技术、原生质体融合、组学研究的应用日益广泛, 微生物菌种改良的新方法和新策略诸如代谢工程、基因组改组和系统生物技术、核糖体工程、表观遗传修饰等逐步发展起来。以下综述了近年来菌种改良相关领域方法和策略特别是首次报道的表观遗传修饰的最新进展。
英文摘要:
      Improvement of the productivity of industrial strains is an important field in micro-biology, because wild-type strains isolated from nature usually produce only a low level of antibiotics. Although random screening and simple rational screening are still effective without using genomic information, they are always time- and labor-consuming. With the broad application of recombinant DNA technology, protoplast fusion and X-omics, novel methods and strategies such as metabolic engineering, genome shuffling, system biology and system biotechnology, ribosome engineering, epigenetic modification are being exploited for the industry microbiology. In this review, we will focus on the progress of these novel methods and strategies for strain improvement in recent years.
赵文婷,邹懿,胡昌华.微生物菌种改良的新方法新策略[J].生物工程学报,2009,25(6):801~805
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