药用昆虫蜣螂对灵芝发酵和抗小鼠肝癌活性的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(No. 30700552) 资助。


Effects of medicinal insect Catharsius molossus on submerged fermentation and in vivo anti-hepatoma activity of Ganoderma lucidum
Author:
Affiliation:

Fund Project:

National Natural Science Foundation of China (No. 30700552).

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    采用液体深层发酵方式, 研究了药用昆虫蜣螂对灵芝细胞生长、关键活性产物发酵动力学和抗小鼠肝癌活性的影响。结果表明, 药用昆虫蜣螂在各添加浓度下对灵芝的细胞生长均无显著促进作用; 但在添加量为5 g/L时, 对灵芝多糖和灵芝三萜的发酵动力学有显著影响(P<0.05), 在发酵第7天时, 灵芝总多糖和总三萜的产量分别达到2.81 g/L 和 539.0 mg/L(对照组分别为2.25 g/L和 428.2 mg/L)。小鼠体内抗肝癌结果表明, 灵芝对照发酵物的抑癌率为41.61%, 灵芝-蜣螂配合物的抑癌率为42.24%; 而补加蜣螂发酵后的灵芝加蜣发酵物的抑癌率高达57.21%, 与灵芝对照发酵物的抑癌率相比, 提高了37.49%。研究表明, 采用昆虫蜣螂补料-分批发酵后, 灵芝发酵物抗小鼠肝癌的活性得到显著增强。

    Abstract:

    We studied the effects of Catharsius molossus (a Chinese medicinal insect) on the cell growth, fermentation kinetics of key bioactive substances and anti-cancer activity of Ganoderma lucidum in submerged fermentation. The results showed that C. molossus at all the tested concentrations had no stimulatory effect on the cell growth. However, addition of C. molossus at 5 g/L lead to significant effects on the fermentation kinetics of polysaccharides and triterpenoids of G. lucidum, and at 7th day in fermentation process, the yields of polysaccharides and triterpenoids reached 2.81 g/L and 539.0 mg/L, respectively, while they were 2.25 g/L and 428.2 mg/L in control. In vivo anti-cancer studies showed that the inhibitory rates of control fermented G. lucidum (CFG) and a combination of water extract from C. molossus and CFG on the developed tumor (Heps) in mice were 41.61% and 42.24%, respectively. Moreover, the inhibitory rate of the G. lucidum fermented with C. molossus (GFC) reached 57.21%, which was enhanced 37.49%, compared to the inhibitory rate of the control fermented G. lucidum. These results suggest that supplementation of C. molossus in submerged fermentation of G. lucidum lead to a significant enhancement of the anti-cancer activity of cultured G. lucidum.

    参考文献
    相似文献
    引证文献
引用本文

刘高强,丁重阳,章克昌,王晓玲. 药用昆虫蜣螂对灵芝发酵和抗小鼠肝癌活性的影响[J]. 生物工程学报, 2009, 25(6): 880-886

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-02-16
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
您是第位访问者
生物工程学报 ® 2024 版权所有

通信地址:中国科学院微生物研究所    邮编:100101

电话:010-64807509   E-mail:cjb@im.ac.cn

技术支持:北京勤云科技发展有限公司