Filamentous microalga Tribonema sp. has the advantages of highly resistance to zooplankton-predation, easy harvesting, and high cellular lipid content, in particular large amounts of palmitoleic acid (PA) and eicosapentaenoic acid (EPA). Therefore, Tribonema sp. is considered as a promising biomass feedstock to produce biodiesel and high-value products. In this work, we studied the effect of different concentrations of nitrogen (NaNO3: 255–3 060 mg/L), phosphorus (K2HPO4: 4–240 mg/L), iron ((NH4)3FeC12H10O14: 0.6–12 mg/L) and magnesium (MgSO4: 7.5–450 mg/L) on the biomass, lipid content, and fatty acid composition of Tribonema sp. FACHB-1786, aiming at enhancing cell lipid productivity. The growth of Tribonema sp. had a positive correlation with the concentration of magnesium, and the maximum biomass of Tribonema sp. (under the condition of 450 mg/L MgSO4) was 8.09 g/L, much greater than those reported in previous studies using the same and other Tribonema species under autotrophic conditions. Different nitrogen concentrations exerted no significant effect on algal growth (P > 0.05), but a higher nitrogen concentration resulted in a greater amount of lipid in the cells. The maximum volumetric productivities of total lipids (319. 6 mg/(L·d)), palmitoleic acid (135.7 mg/(L·d)), and eicosapentaenoic acid (24.2 mg/(L·d)) of Tribonema sp. were obtained when the concentrations of NaNO3, K2HPO4, (NH4)3FeC12H10O14, and MgSO4 were 765 mg/L, 80 mg/L, 6 mg/L, and 75 mg/L, respectively. This study will provide a reference for substrate optimization for Tribonema sp. growth and lipid production.
黄丝藻矿质元素藻油棕榈油酸二十碳五烯酸微藻培养Tribonema sp.mineral nutrientsalgal oilpalmitoleic acideicosapentaenoic acidmicroalgal culture国家开发投资有限公司项目SDICMBC-00003国家开发投资有限公司项目(No. SDICMBC-00003)资助State Development & Investment CorporationSDICMBC-00003State Development & Investment Corporation (No. SDICMBC-00003)
Effects of different initial NaNO3 concentrations on the accumulation of biomass (A), lipid (B) and fatty acids contents (C) of Tribonema sp. FACHB-1786. a: 4 d; b: 8 d; c: 12 d.
Effects of different initial K2HPO4 concentrations on the accumulation of biomass (A), lipid (B) and fatty acids contents (C) of Tribonema sp. FACHB-1786.
Effects of different initial (NH4)3FeC12H10O14 concentrations on the accumulation of biomass (A), lipid (B) and fatty acids contents (C) of Tribonema sp. FACHB-1786.
Effects of different initial MgSO4 concentrations on the accumulation of biomass (A), lipid (B) and fatty acids contents (C) of Tribonema sp. FACHB-1786.
Effects of initial concentrations of different nutrients on total lipits volumetric productivities of Tribonema sp. FACHB-1786 FACHB-1786. (A) NaNO3.(B) K2HPO4. (C) (NH4)3FeC12H10O14. (D) MgSO4.
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