Actinomycetes are a class of Gram-positive bacteria that can produce primary metabolites such as amino acids and secondary metabolites such as antibiotics. Actinomycetes are widely used in food, pharmaceutical, additive and cosmetic industries. In addition, a few actinomycetes, such as Mycobacterium, are pathogen that can cause human, animal and plant diseases. Leucine-responsive regulatory protein (Lrp) is a category of global transcriptional regulator involved in amino acid metabolism and its relevant metabolic processes. They are capable of responding to a variety of amino acids and participating in the regulation of multiple physiological processes in microbial cells, such as amino acid metabolism and transport, central metabolism, bacterial persistence and virulence, etc. This paper summarizes the biological functions of Lrp in actinomycetes, and reviews the research advance of regulatory mechanism of Lrp from different actinomycetes, especially Lrp from Streptomyces coelicolor and Saccharopolyspora erythraea studied.