[Objective] To study the effects of different probiotics on mice obesity based on the interaction between microbiota and host metabolism. [Methods] In total 50 C57BL/6J male mice were equally divided into 10 groups and fed with normal feed, high-fat feed and high-fat feed with eight different probiotics compositions (5 billion CFU/mice). All mice were continuously fed for nine weeks and weighted weekly. Fasting blood glucose, GTT, blood fat indexes and organ weight were measured at last week. Microbiota total DNA was extracted from cecum contents and sequenced by 16S rDNA sequencing. [Results] Some probiotic compositions accelerated mice body weight accumulation, whereas some probiotic compositions reduced obesity, decreased visceral fat and relieved hyperlipemia. Compared to the normal feed group, Firmicutes/Bacteroidetes (F/B) ratio was 22.8-fold in accelerated body weight group fed with Danisco probiotic, and none Akkermansia muciniphila (Akkermansia) was detected, while F/B ratio was similar and the Akkermansia content was 0.5% (half of normal feed group) in reduced obesity group fed with Junlading probiotic. [Conclusion] Some probiotics influence mice body weight and metabolism, but different compositions may lead to opposite effects. Specific probiotic composition improves obesity and hyperlipemia possibly by its selected strain characteristics and relative ratio through decreasing F/B ratio and increasing Akkermansia level in gut microbiota. This study contributes to developing metabolism-improving probiotic product in the future.