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酸胁迫下琥珀酸放线杆菌的生理及转录应答
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国家自然科学基金(21604032);“十二五”农村领域国家科技计划课题(2015BAD15B04);中央高校基本科研业务费专项资金(JUSRP116031);江南大学工业生物技术教育部重点实验室开放课题(KLIB-KF201501)


Physiological and transcriptional responses of Actinobacillus succinogenes to acid stress
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    摘要:

    [目的]通过琥珀酸放线杆菌Actinobacillus succinogenes CGMCC1593对酸胁迫的生理应答和转录组学分析,探究琥珀酸放线杆菌酸胁迫的机制。[方法]测定不同pH对细胞生长、H+-ATPase、细胞内pH的影响;测定酸胁迫前后细胞膜和谷氨酸脱氢酶的变化、谷氨酸对琥珀酸放线杆菌生长的影响;通过RNA-seq测序分析酸胁迫条件下的差异表达基因。[结果]随pH值的降低,细胞生长受抑制,H+-ATPase的活性下降。pH 4.7酸胁迫后,细胞膜受到严重损伤,谷氨酸对酸胁迫后的细胞有保护作用,GDH酶活响应酸胁迫后略有增加。酸胁迫后,39个基因差异表达较为显著,其中49%基因属于应激蛋白、转运蛋白,小部分基因与代谢相关。[结论]本文探究了琥珀酸放线杆菌酸胁迫下的生理及转录应答,研究结果可为寻找增强琥珀酸放线杆菌耐酸性策略提供参考。

    Abstract:

    [Objective] We explored by physiological and transcriptional responses of Actinobacillus succinogenes CGMCC1593 to acid stress. [Methods] We studied the effects of different initial pH on cell growth, activity of H+-ATPase and intracellular pH before and after acid stress as well as the protection mechanism of glutamate on CGMCC1593. The transcriptomics technique was used to explore the differentially expressed genes under acid stress. [Results] Cell growth was inhibited and the activity of H+-ATPase decreased with the decrease of initial pH. After acid stress at pH 4.7, the cell membrane was severely damaged. Glutamate had a protective effect on the cells under acid stress. In total 39 genes were differently expressed altogether under acid stress, of which 49% were stress and transport proteins, and a small part was related to metabolism. [Conclusion] Understanding the physiological and transcriptional responses of Actinobacillus succinogenes to acid stress provides references for improving acid resistance of Actinobacillus succinogenes.

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张群,陈鹏程,郑璞. 酸胁迫下琥珀酸放线杆菌的生理及转录应答. 微生物学报, 2018, 58(7): 1255-1265

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  • 收稿日期:2017-08-15
  • 最后修改日期:2017-10-18
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  • 在线发布日期: 2018-07-05
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