科微学术

微生物学报

利用蛋白质组学研究新德里金属b-内酰胺酶1对鲍曼不动杆菌的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(21571082);广州市科技计划(201607010228)


Comparative proteomics reveals the role of NDM-1 in Acinetobacter baumannii
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    [目的]比较临床分离的亲缘关系近的多药耐药鲍曼不动杆菌MDR-ZJ06(blaNDM-1-)和ABC3229(blaNDM-1+)的差异蛋白质组,以期发现新德里金属b-内酰胺酶1(New Delhimetallo-β-lactamase-1,NDM-1)对鲍曼不动杆菌生长代谢的影响。[方法]利用2-DE联合MALDI-TOF MS/MS技术鉴定差异表达蛋白,并在GO注析的基础上,对差异蛋白进行通路分析、功能分类和富集分析,并作出蛋白与蛋白相互作用网络。[结果]发现ABC3299相对于MDR-ZJ06有51个差异表达蛋白,其中11个蛋白表达上调,40个蛋白表达下调,并且这些差异蛋白主要涉及降低碳代谢、氨基酸代谢、脂肪酸代谢和细胞壁合成,增加铁离子转运系统形成。[结论]这个结果揭示了NDM-1可能是通过减缓细菌自身的代谢,增加自身铁的摄取使细菌机体系统地抵抗抗生素从而达到耐药。

    Abstract:

    [Objective] In order to investigate how NDM-1 affect the metabolism of A. baumannii, we analyzed the differentially expressed proteins (DEPs) between clinically isolated multidrug-resistant A. baumannii MDR-ZJ06 (blaNDM-1-) and ABC3229 (blaNDM-1+) which have a close genetic relationship. [Methods] We used 2-DE coupled with MALDI-TOF MS/MS to analyze the differentially expressed proteins (DEPs) between A. baumannii MDR-ZJ06 and ABC3229. Based on GO annotations, we analyze DEPs by KEGG, Function classification, enrichment analysis and protein-protein interaction network. [Results] We found that there were 51 DEPs, including 11 up-regulated proteins and 41 down-regulated proteins in ABC3229. These proteins were predominantly involved in reducing carbohydrate metabolism, amino acid metabolism, fatty acid metabolism, cell wall synthesis, and increasing of iron transport system formed. [Conclusion] These results uncover the effect of NDM-1 on bacteria may be resistant to antibiotics by slowing down the metabolism of bacteria and increasing the uptake of iron, thereby the bacteria will systemic resistance antibiotics.

    参考文献
    相似文献
    引证文献
引用本文

曾观娣,徐倩,刘婉婷,阳小燕,孙雪松. 利用蛋白质组学研究新德里金属b-内酰胺酶1对鲍曼不动杆菌的影响. 微生物学报, 2018, 58(5): 804-816

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-05-31
  • 最后修改日期:2017-06-29
  • 录用日期:
  • 在线发布日期: 2018-05-06
  • 出版日期: