Microbes own structural diversity and functional diversity, and their ecological behavior is regulated by various signal molecules. Among the signal factors, a most typical one is quorum sensing (QS). QS is a form of intercellular communication within microbial populations and occurs in a density-dependent manner. QS substance can result in down-stream changes in gene regulation and modulation of bioluminescence, virulence, biofilm formation and secondary metabolite production. Corresponding to QS, quorum sensing inhibitors (QSI) has also gradually been noticed in recent years. Previously, QSI is widely applied in medicine, agriculture and environmental sectors. They can exhibit multiple biological functions by regulate QS signal, such as reducing bacterial pathogenicity, interfering biofilm formation, blocking signal receptors and influencing QS-based cascading effects. We reviewed the latest advances of QSI, including their sources, properties, and mechanisms of action. In addition, the ecological applications of QSI in aquaculture, marine antifouling, sewage treatment and marine drug development have also been discussed, to supply novel strategies for developing QSI compounds. We provide herewith a reference for readers to understand QSI roles and using marine source more efficiently.