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山东地区盐碱土花生种子际土壤微生物群落结构的研究
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“十三五”国家科技支撑计划(2014BAD11B04-03);山东省现代农业产业技术体系创新团队岗位专家(花生) (SDAIT-04-06);山东省农业重大应用技术创新课题(2014);山东省自主创新专项基金(2014ZZCX07401-12)


Diversity of microbial community structure in the spermosphere of saline-alkali soil in shandong area
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    摘要:

    [目的]以不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤为实验对象,探讨花生种子在吸水膨胀与萌发过程中,不同类型盐碱土对种子际土壤微生物多样性变化的影响。[方法]采集不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤,通过对各样品中细菌的16S rRNA基因的V3-V4区进行PCR扩增,利用Illumina Hiseq高通量测序技术对12份V3-V4高变区PCR产物进行测序,并对测序数据进行生物信息学分析。[结果](1) 盐碱土壤的种子际细菌群落多样性高于非盐碱土壤,且以东营青坨滨海盐土种子际土壤细菌群落多样性较高。(2) 不同类型土壤样本微生物群落结构在纲水平存在明显差异。4种土壤类型种子际土壤细菌共分属于6个菌纲,分别为Proteobacteria、Actinobacteria、Actinobacteria、Bacteroidetes、Acidobacteria和Firmicutes菌纲,并均以Proteobacteria和Actinobacteria菌纲为主要菌纲。全样本菌落结构分析结果表明,4种类型土壤中不同吸胀时间内种子际微生物菌落在门、属水平上的类型和丰度差异最为显著(P<0.05)。(3) beta多样性分析和各样本遗传距离(phylogenetic distances)聚类树图分析表明,4个土壤类型的12个土壤样本种子际土壤中微生物群落均可聚为2大类。[结论]土壤含盐量越高其种子际土壤细菌群落多样性较高。不同类型土壤样本微生物群落结构在纲水平存在明显差异,以Proteobacteria和Actinobacteria菌纲为主要菌纲。种子吸胀萌发时间影响种子际微生物菌落在门、属水平上的类型和丰度,但对相同土壤类型样本间遗传距离无影响。

    Abstract:

    [Objective] Three soil types in different salt contents were taken as the experiment objectives. We evaluated the effect of various saline alkali soil types on diversity of bacterial community structure in spermosphere soil during water absorption and germination of peanut seeds.[Methods] The V3-V4 region of 16S ribosomal RNA genes was amplified using PCR, and the PCR products were then analyzed using Illumina high-throughput sequencing technology. [Results] (1) The diversity of soil bacterial community in saline alkali soil was higher than that in non-saline alkali soil. Especially, the highest diversity was in spermosphere soil from Qingtuo. (2) The microflora structures in different soils were distinct at the class level. Soil bacteria in four samples were classified into six classes, including Proteobacteria, Actinobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes. Proteobacteria and Actinobacteria groups were dominant in colonies. The analysis of whole samples colony structure showed that the difference of type and abundance at phylum and genus level during different adsorption time was most significant (P<0.05). (3) The analysis of beta diversity and phylogenetic distances of constructed phylogenetic trees revealed that the sequenced clones fell into two major groups within the domain bacteria.[Conclusion] The diversity of bacteria community compositions in the high salt content soil was higher. There were obvious differences in microbial community structure of different soil types at class level, primarily in the Proteobacteria and Actinobacteria. The type and abundance of microbial colonies at both phylum and genus levels were affected by the seed germination time. However, there was no influence on the genetic distance between the samples from the same soil type.

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张智猛,慈敦伟,张冠初,丁红,杨吉顺,戴良香,张岱. 山东地区盐碱土花生种子际土壤微生物群落结构的研究. 微生物学报, 2017, 57(4): 582-596

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  • 收稿日期:2016-08-25
  • 最后修改日期:2016-11-09
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  • 在线发布日期: 2017-03-31
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