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微生物学报

植物病原黄单胞菌退出群体感应生理状态的分子机制和生物学意义
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国家重点研发计划(2018YFA0901901);国家自然科学基金(31972231,31772121)


Molecular mechanisms and biological significance of the naturally occurring turnover of DSF-family quorum sensing signal in the phytopathogen Xanthomonas
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    摘要:

    黄单胞菌是一类引起多种作物病害的病原细菌总称。它们利用自身产生的DSF(Diffusible signaling factor)-家族群体感应(quorum sensing,QS)信号分子感应群体密度,调控致病相关基因的表达。当黄单胞菌培养达到对数生长后期时,培养体系中DSF信号分子浓度迅速降低,呈现一种典型的群体感应信号反转(turnover)现象。编码脂酰辅酶A连接酶的基因rpfB在黄单胞菌生长后期表达量显著提高,敲除rpfB导致DSF生物合成显著提高,因此,RpfB参与DSF-家族QS信号分子的降解,在群体感应后期引导黄单胞菌退出群体感应状态。DSF信号通过RpfC/RpfG双组分系统、环二鸟苷酸和全局性转录因子Clp负调控rpfB的表达。DSF群体感应信号关键降解基因rpfB存在于多种细菌中,表明该信号反转机制相对保守,但其调控的生物学功能因菌而异。

    Abstract:

    Xanthomonas represents a range of bacterial species that cause diseases in a variety of crops. They produce and use DSF (Diffusible signaling factor)-family quorum sensing (QS) signal to sense cell population to regulate the expression of virulence genes. At post-logic growth phase, the levels of DSF-family signal decreased rapidly, indicating a naturally occurring signal turnover phenomenon in Xanthomonas. The expression levels of rpfB that encodes a putative fatty acyl CoA ligase, significantly increased at logarithmic growth phase. Deletion of rpfB significantly increased the production of DSF-family signal. Therefore, RpfB is involved in the degradation of DSF-family signals, including Xcc to exit from the quorum sensing phase at post-QS phase. DSF-family signal negatively regulates rpfB expression via rpfC/rpfG two-component signaling system, cyclic di-GMP and the global regulator Clp. The RpfB-dependent naturally DSF turnover system is present in a range of bacterial species, representing a conserved mechanism for QS signal turnover. However, the RpfB-regulated biological roles differ from species to species.

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宋凯,郭晓春,陈博,何亚文. 植物病原黄单胞菌退出群体感应生理状态的分子机制和生物学意义. 微生物学报, 2021, 61(1): 68-76

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  • 收稿日期:2020-09-17
  • 最后修改日期:2020-11-02
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  • 在线发布日期: 2021-01-12
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