| 灰树花子实体与发酵菌丝体挥发性化合物分析 |
| Analysis of volatile compounds in fruiting bodies and submergedly cultured mycelia of Grifola frondosa |
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| 中文关键词:灰树花，挥发性化合物，顶空固相微萃取，气相色谱-质谱分析 |
| 英文关键词:Grifola frondosa, volatile compounds, headspace solid-phase microextraction, gas chromatography-mass spectrometry analysis |
| 基金项目:江苏省自然科学基金（No. BK2010142）；国家“863”重点项目子课题（No. 2007AA021506）；教育部新世纪人才支持计划（No. NCET-07-0380） |
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| 中文摘要: |
| 分别采用固体栽培法和液体发酵法对同一灰树花菌株进行培养，获得灰树花子实体和发酵菌丝体，并采用顶空固相微萃取（HS-SPME）和气相色谱-质谱（GC-MS）分析技术对子实体和菌丝体的挥发性化合物进行了分析比较。结果表明，灰树花子实体和发酵菌丝体分别含有62种和94种挥发性物质，其中37种为相同物质，分别占总挥发性物质的86.81%和84.28%。灰树花子实体中含有醇（14种）、酮（13种）、醛（12种）、酸（7种）、酯（5种）等物质，主要以酯类（42.80%）、醛类（35.14%）物质为主，其中乙酸乙酯的相对含量达到了42.57%。发酵菌丝体中主要含有醛（22种）、酮（17种）、醇（16种）、酯（13种）、酸（12种）等挥发性物质，其中醛类、酯类、酸类、醇类分别占47.0%、10.90%、7.48%和5.94%。异戊醛和地衣酚的含量分别达到了23.31%、15.41%。 |
| 英文摘要: |
| Fruiting bodies and mycelia of Grifola frondosa were produced by solid-state and submerged cultures, respectively. The volatile compounds of fruiting bodies and mycelia were analyzed by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that fruiting bodies and mycelia of Grifola frondosa, respectively, contained 62 and 94 volatile compounds. Hereinto, 37 compounds in both fruiting bodies and mycelia accounted for 86.81% and 84.28% of total volatile substances, respectively. The fruiting bodies mainly contained alcohol (14), ketones (13), formaldehyde (12), acid (7), esters (5), and so on. Esters (42.80%) and aldehydes (35.14%) were the main volatile compounds of fruiting bodies, and the relative content of ethyl acetate was 42.57%. The mycelia mainly contained formaldehyde (22), ketones (17), alcohol (16), esters (13), acid (12) and so on, of which aldehydes, esters, acids and alcohols, respectively, accounted for 47.0%, 10.90%, 7.48% and 5.94%. The content of isovaleraldehyde and lichen phenol reached 23.31% and 15.41%, respectively. |
| 杨生兵,陆震鸣,耿燕,许泓瑜,徐国华,史劲松,许正宏. 灰树花子实体与发酵菌丝体挥发性化合物分析[J]. 菌物学报, 2013, 32(1): 103-113 |
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