| 粗皮侧耳栽培种EST-SSR分子标记的开发应用及初级核心种质库的构建 |
| Development of EST-SSR markers in Pleurotus ostreatus and construction of primary core collection |
| |
| 中文关键词:粗皮侧耳，EST-SSR分子标记，初级核心种质库，有效等位基因，多态性位点 |
| 英文关键词:Pleurotus ostreatus, EST-SSR marker, primary core collection, effective alleles, polymorphism sites |
| 基金项目:国家食用菌产业技术体系建设项目（No. CARS-24）；山东省优秀中青年科学家科研奖励基金：侧耳栽培种核心种质及其指纹图谱库的构建研究；山东省农业良种工程项目（No. 2011LZ006-04）；山东省科技发展计划项目：基于宏基因组高通量测序的食用菌病害检测与监测技术及相关基因的研究；农业科技成果转化资金项目：城郊食用菌产业化开发 |
|
| 摘要点击次数: 2909 |
| 全文下载次数: 1709 |
| 中文摘要: |
| 根据已知的粗皮侧耳、灵芝、香菇EST序列开发出9条粗皮侧耳EST-SSR引物、58条灵芝EST-SSR引物和35条香菇EST-SSR引物，从中筛选出4条多态性好、条带清晰的引物，对选取的185株粗皮侧耳栽培种进行扩增分析。共扩增得到59条带，每个引物扩增条带数为12–19条，扩增片段在100–1,100bp之间。根据扩增结果构建了一个含47株粗皮侧耳栽培种的初级核心种质库P-core 47。分析得出P-core 47占原群体样品数25%，占资源库的13%，有效等位基因数和多态性位点占有率与原群体一致，多样性指数均值比原群体高0.12747，在原群体的低频率等位基因有所增强。P-core 47内菌株来源广泛，基本已覆盖各个栽培地区，统计的质量性状和数量性状均可在其中找到代表性菌株。表明了用EST-SSR分子标记构建粗皮侧耳栽培种初级核心种质库的可行性。 |
| 英文摘要: |
| Based on the EST sequences published on NCBI, 9 Pleurotus ostreatus EST-SSR primer pairs, 58 Ganoderma lucidum EST-SSR primer pairs and 35 Lentinula edodes EST-SSR primer pairs were designed. Among these, 4 of good polymorphisms and clear strips were selected to perform the amplification analysis for the cultivation of the chosen 185 strains of Pleurotus ostreatus. After amplification, 59 alleles were generated, and there were 12–19 alleles for each primer, with the fragment sizes between 100bp and 1,100bp. A primary core collection, P-core 47, composed of 47 accessions was constructed based on the amplification results. After analysis, P-core 47 accounts for 25% in the original sample pool, 13% in the oringinal resource pool; as for the number of effective alleles and occupation ratio of polymorphism sites, consistency was preserved between P-core 47 and the original pool; in addition, compared to the original pool, diversity index of P-core outperformed by 0.12747, and alleles used to be of low frequency in the original pool were strengthened as well. The strains in P-core 47 are widely-ranged, basically available in most of the cultivation regions; you can find representative alleles of both qualitative and quantitative characters in this collection. This showed the feasibility of the adoption of EST-SSR markers to construct a primary core collection of the Pleurotus ostreatus. |
| 张雪梅,姚强,宫志远,高兴喜,李瑾,刘晓,韩建东,任鹏飞,万鲁长,任海霞. 粗皮侧耳栽培种EST-SSR分子标记的开发应用及初级核心种质库的构建[J]. 菌物学报, 2013, 32(1): 64-80 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|