| 蚜科专化菌努利虫疠霉菌种超低温储存 |
| Cryopreservation of aphid-specific pathogen Pandora nouryi |
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| 中文关键词:超低温储存，努利虫疠霉，萨氏培养基，冷冻保护剂，毒力 |
| 英文关键词:cryopreservation, Pandora nouryi, Sabouraud dextrose broth, cryoprotectant, virulence |
| 基金项目:华侨大学基本科研业务费专项基金（No. JB-ZR1116）；华侨大学高层次人才科研启动（No. 09BS402）；浙江省教育厅科研项目（No. Y201119481）；浙江农林大学科研启动项目（No. 2010FR065） |
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| 中文摘要: |
| 虫霉目真菌的活力对超低温储存较为敏感。储存法在大范围应用前，需对储存效果进行详细评估。将蚜科专化菌努利虫疠霉以初级分生孢子形式（2－3′105个孢子/mL）在-80℃超低温存储12个月。结果显示日常用于培养该真菌的含0.1%乳化芝麻油的萨氏培养基作为超低温保护基质能有效地储存努利虫疠霉孢子，比常见的冷冻保护剂如二甲亚砜和甘油的效果好。孢子悬液经解冻和培养后可获得最多的生物量，而且菌种保持了较高的生长速率。更重要的是，萨氏培养基的主要成分4%葡萄糖、1%蛋白胨和1%酵母粉在低温存储过程中发挥了协同作用，能保 |
| 英文摘要: |
| The viabilities of entomophthoralean fungi are sensitive to cryopreservation procedures. The efficacy of preservation methods for these fastidious fungi should be evaluated in detail before extensive application. In this study, the primary spores (2－3′105conidia/mL) of an aphid obligate pathogen Pandora nouryi was preserved at -80℃ for 12 months. The routine culture medium of Sabouraud dextrose broth plus 0.1% emulsified sesame oil (SDBS) was selected for culture storation, due to more effective in protecting the fungal spores than ordinary cryoprotectants such as dimethylulphoxide and glycerol. The highest biomass and growth rate were obtained after the preserved spore suspensions were thawed and incubated. Moreover, 4% glucose, 1% peptone and 1% yeast extracts in the liquid culture played cooperative roles in preserving the isolate under ultracold condition, and the virulence retained stable. After one year preservation, the median lethal concentration of the isolate against Myzus persicae nymphs was estimated as 3.3conidia/mm2 by analysis of multiple-dose bioassay data, consistent with that in the previous repeated report. In a word, -80℃ cryopreservation can be accepted as a good preservation technique for entomophthoralean fungi, and Sabouraud dextrose broth is more suitable as cryoprotectantive medium for P. nouryi than 10% glycerol solution. |
| 周湘,冯明光,黄志宏. 蚜科专化菌努利虫疠霉菌种超低温储存[J]. 菌物学报, 2012, 31(2): 285-291 |
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