| 耐热真菌的分类鉴定及rDNA-ITS系统发育分析 |
| Thermotolerant fungi and their phylogenetic analyses based on rDNA-ITS sequences |
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| 中文关键词:高温真菌，中国新记录种，NJ系统发育树 |
| 英文关键词:fungi in high temperature, new records for China, NJ phylogeny tree |
| 基金项目:国家自然科学基金“高温真菌多样性与分类研究”（No. 30870006） |
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| 中文摘要: |
| 分别从山东、山西、河北、陕西、河南、云南、江西、海南、新疆、内蒙古、甘肃、广州等地实地采集堆肥、培养料、草堆等标本600多份，分离鉴定出耐热真菌28种，其中包括2个中国新记录种，沙生梭孢壳Thielavia arenaria和丰孢木霉Trichoderma saturnisporum，其余26个为中国已知种。在形态分类的基础上，对所分离鉴定的28株耐热真菌及购买的3株耐热真菌，进行rDNA-ITS序列测定，所测序列片段长度为554－895bp。将所测的31个和从GenBank下载的15个耐热真菌的rDNA-ITS序列构建NJ系统发育树，该树较好地显示了耐热真菌的系统进化关系，有力地支持了形态学的观点。 |
| 英文摘要: |
| More than 600 specimens of thermotolerant fungi were collected from 12 provinces in China, and 28 species were identified. Among them, Thielavia arenaria and Trichoderma saturnisporum are newly recorded species for China. rDNA-ITS sequencing was performed of the 28 species of thermotolerant fungi and additional 3 isolates purchased from China General Microbiological Culture Collection Center. The length of sequenced fragments were 554－895bp. Neighbor-joining phylogeny tree of rDNA-ITS sequences of the 31 isolates sequenced and 15 isolates downloaded from GenBank was used to reveal the phylogenetic relationships of thermotolerant fungi. The result of the phylogenetic analyses supports the species delimitation based on morphological characteristics. |
| 梁茵,王芳,李安娜,李多川. 耐热真菌的分类鉴定及rDNA-ITS系统发育分析[J]. 菌物学报, 2011, 30(4): 542-550 |
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