| 色钉菇粗多糖对小鼠DA能神经元MPTP损伤的保护作用 |
| Protective effects of crude polysaccharides from Chroogomphus rutilus on dopaminergic neurons impaired by MPTP in mice |
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| 中文关键词:帕金森病，黑质，行为，保护作用，抗氧化 |
| 英文关键词:Parkinson’s disease, substantia nigra, behavior, protective effects, antioxidant |
| 基金项目:河北师范大学应用开发基金项目（No. L2009K03）；河北省自然科学基金（No. C2008000155）；河北省教育厅重点研究计划项目（No. ZH2007107） |
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| 中文摘要: |
| 以C57BL/6J小鼠为实验材料，研究色钉菇粗多糖对多巴胺能神经元损伤的保护作用，并用体外实验方法测定其抗氧化活性。用MPTP（1-甲基-4-苯基-1,2,3,6-四氢吡啶）方法复制了帕金森病小鼠模型，模型复制前，预先给予小鼠100mg/kg、200mg/kg、400mg/kg不同剂量的色钉菇粗多糖，通过行为学检测方法和免疫组化技术观察小鼠的行为变化以及黑质中多巴胺能神经元的数量变化；用抑制小鼠肝脂质过氧化能力的方法测定色钉菇粗多糖的抗氧化活性。结果显示，预先给予200mg/kg和400mg/kg剂量的色钉菇粗多糖能显著地改善帕金森病模型小鼠的行为症状，并能显著地降低MPTP所致的小鼠多巴胺能神经元的凋亡，这可能与该多糖的抗氧化活性有关。提示色钉菇多糖有开发成防治帕金森病药物的应用前景。 |
| 英文摘要: |
| To investigate the protective effects of crude polysaccharides from Chroogomphus rutilus on doaminergic neurons impaired by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6J mice were investigated, and to analyse the antioxidant activity of the crude polysaccharides in vitro was analysed. The mouse model of Parkinson’s disease was duplicated by using intraperitoneal injection of MPTP. The mice were pretreated through gavage with different dosages of crude polysaccharides (100mg/kg, 200mg/kg and 400mg/kg/day) from Chroogomphus rutilus and then the behavior and the quantitative changes of substantia nigra dopaminergic neurons were observed in these mice by behavioral detection and immunohistochemical methods. The antioxidant activity of the crude polysaccharides was measured by using the method of inhibition of lipid peroxidation in mice. The results showed that pretreatment with crude polysaccharides from Chroogomphus rutilus at dosages of 200mg/kg and 400mg/kg significantly improved the behavioral symptoms of the mice with Parkinson’s disease and thereby effectively protected dopaminergic neurons of the mice from apoptosis induced by MPTP. The protected effects may relate to the antioxidant activity of polysaccharides. This indicates that the polysaccharides from Chroogomphus rutilus are one of the possible candidates for drug discovery to treat Parkinson’s disease. |
| 张雪倩,孙红,王立安,王艳芹,刘川,白云,李洁,管振龙. 色钉菇粗多糖对小鼠DA能神经元MPTP损伤的保护作用[J]. 菌物学报, 2011, 30(1): 77-84 |
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