| 双孢蘑菇分解基质能力退化的DDRT-PCR分析 |
| DDRT-PCR analysis of the substrate-decomposing capability of degenerate strains of Agaricus bisporus |
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| 中文关键词:食用菌，mRNA，差异DNA片段，克隆，多糖类基质 |
| 英文关键词:edible fungus, mRNA, differential DNA fragment, cloning, polysaccharide substrate |
| 基金项目:国家自然科学基金（No. 30871769） |
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| 中文摘要: |
| 对4种不同液体培养基培养的双孢蘑菇Agaricus bisporus CGMCC No. 0214及其分解基质能力退化菌株0214-3、0214-5进行了24对引物组合的mRNA差异显示（DDRT-PCR）分析，结果发现8对引物组合扩增出可重复的差异，并克隆了9条差异片段。序列分析结果显示9条差异片段代表了5个基因的差异，它们与不同生物的ATP结合盒式运输亚家族A某蛋白、碳水化合物酯酶家族4蛋白、几丁质脱乙酰基酶、乙酰木聚糖酯酶II及其他一些未知蛋白具有较高的同源性。本研究结果为进一步进行双孢蘑菇分解基质能力退化的分子机理研究奠定了良好的基础。 |
| 英文摘要: |
| mRNA differential display reverse transcription-PCR (DDRT-PCR) analysis with 24 pairs of primers was used to analyze mRNA differences between the wild type of Agaricus bisporus strain CGMCC No. 0214 and its substrate-decomposing ability degeneration strains 0214-3 and 0214-5 cultured in 4 kinds of different liquid media. As a result, 8 pairs of primers showed repeatable differences, and 9 special fragments were cloned. Sequencing analysis showed that 9 special fragments represented 5 different genes. These genes have high homologies with an ATP-binding cassette transporter subfamily A protein, carbohydrate esterase family 4 protein, chitin deacetylase, acetyl xylan esterase II and some other hypothetical proteins of several kinds of organisms. The results of this study provided a basis for further study of the molecular mechanism of substrate degradation ability degeneration of A. bisporus. |
| 陈美元,王泽生,李洪荣,廖剑华,谢宝贵. 双孢蘑菇分解基质能力退化的DDRT-PCR分析[J]. 菌物学报, 2010, 29(5): 707-712 |
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